High-throughput compound evaluation on 3D networks of neurons and glia in a microfluidic platform
نویسندگان
چکیده
With great advances in the field of in vitro brain modelling, the challenge is now to implement these technologies for development and evaluation of new drug candidates. Here we demonstrate a method for culturing three-dimensional networks of spontaneously active neurons and supporting glial cells in a microfluidic platform. The high-throughput nature of the platform in combination with its compatibility with all standard laboratory equipment allows for parallel evaluation of compound effects.
منابع مشابه
Controllable organization and high throughput production of recoverable 3D tumors using pneumatic microfluidics.
Three-dimensional tumor culture methods offer a high degree of biological and clinical relevance to in vitro models as well as cancer therapy. However, a straightforward, dynamic, and high-throughput method for micro-manipulation of 3D tumors is not yet well established. In this study, we present a novel and simple strategy for producing biomimetic 3D tumors in a controllable, high throughput m...
متن کاملCircular compartmentalized microfluidic platform: Study of axon-glia interactions.
We describe a compartmentalized circular microfluidic platform that enables directed cell placement within defined microenvironments for the study of axon-glia interactions. The multi-compartment platform consists of independent units of radial microchannel arrays that fluidically isolate somal from axonal compartments. Fluidic access ports punched near the microchannels allow for direct pipett...
متن کاملFluorescent Contrast agent Based on Graphene Quantum Dots Decorated Mesoporous Silica Nanoparticles for Detecting and Sorting Cancer Cells
Background and Objectives: The inability of classic fluorescence-activated cell sorting to single cancer cell sorting is one of the most significant drawbacks of this method. The sorting of cancer cells in microdroplets significantly influences our ability to analyze cancer cell proteins. Material and Methods: We adapted a developed microfluidic device as a 3D in vitro model to sorted MCF-7 c...
متن کاملA label-free and high-throughput separation of neuron and glial cells using an inertial microfluidic platform.
While neurons and glial cells both play significant roles in the development and therapy of schizophrenia, their specific contributions are difficult to differentiate because the methods used to separate neurons and glial cells are ineffective and inefficient. In this study, we reported a high-throughput microfluidic platform based on the inertial microfluidic technique to rapidly and continuou...
متن کاملA high-throughput photodynamic therapy screening platform with on-chip control of multiple microenvironmental factors.
We present a novel high-throughput microfluidic platform that enables the evaluation of the anticancer efficacy of photodynamic therapy (PDT) drugs over multiple microenvironmental factors. PDT is uniquely complex, originating from its dependence on three separate but essential elements: drug (also called photosensitizer), oxygen, and light. Thus, obtaining a reliable evaluation of PDT efficacy...
متن کامل